The Potential Protective Effect of Ferulic Acid against Gamma Irradiation Induced Ovarian Failure in Rats
Salma
Abdel Fattah
Department of Drug Radiation Research, National Center for Radiation Research
and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
author
Heba
Mohmed
Department of Drug Radiation Research, National Center for Radiation Research
and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
author
Marwa
Mohamed
Department of Drug Radiation Research, National Center for Radiation Research
and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
author
text
article
2019
eng
This study aims to evaluate the radioprotective effect of Ferulic acid (FA) against ovarian damage in premature female rats. Four groups of female rats were used (ten rats in each group). Group (1): Control, Group (2): Irradiation (IRR) (rats were exposed to 4Gy of whole body gamma irradiation as a single dose), Group (3): FA (rats received 50mg/kgb.wt of Ferulic acid orally for two weeks) and Group (4): FA+IRR (rats received Ferulic acid as group 3 then exposed to 4Gy as group2). The rats were sacrificed after 24h of irradiation. The level of FSH, LH, and E2were estimated in the serum. The level of H2O2 and GPx activity and the gene expression of cytochrome C, P53 and caspase3 were evaluated in ovarian tissue. Histopathological examination of the ovary and uterus tissues was performed. The results revealed that gamma irradiation caused an elevation in FSH, LH, H2O2, cytochrome C, P53 and caspase3 and a decline in E2 and GPx. The administration of FA prior gamma irradiation led to an improvement in female hormones, H2O2, GPx and apoptotic markers and matched the histopathological examination results. It could be concluded that Ferulic acid may exert aradioprotective effect against irradiation induced apoptosis in the ovary of rats and alteration in the level of serum female hormones.
Egyptian Journal of Radiation Sciences and Applications
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
1110-0303
32
v.
1
no.
2019
1
12
https://ejrsa.journals.ekb.eg/article_28076_bd3ad08b1773a67e59ef657f64f40290.pdf
dx.doi.org/10.21608/ejrsa.2019.6608.1058
Comparative Study of Gamma Radiation and Prednisolone Induced Submandibular Salivary Gland Oxidative Damage
Salwa
Ahmed
Health Radiation Research Department, National Centre for Radiation Research and
Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
author
text
article
2019
eng
The most pronounced salivary dysfunction occurs in patients taking medications that interfere with salivary secretory processes and those who have received therapeutic irradiation. The aim of the present study is to investigate the effect of gamma radiation and prednisolone treatment on submandibular salivary glands. Forty eight female Albino rats weredivided into four groups. Group C serves as control; group P was treated with Depo-Medrol; group R was exposed to whole body gamma radiation and group PR is similar to the previous one in addition, it has received Depo-Medrol. The submandibular glands were obtained three and seven days post-irradiation. Gamma-irradiation induced vacuolization of all gland compartments, acinar atrophy and degeneration of striated ducts and granular convoluted tubules. In group P, the acini lost their outline with shrinkage of some acini. The striated ducts represented variable degree of degeneration, lumen narrowing. Nuclear fragmentation was detected in duct and granular convoluted tubule cells. All previously mentioned manifestations were detected in an exaggerated manner in group PR. Thetotal glutathione content and glutathione-peroxidase activity significantly decreased in all experimental groups. Activity of superoxide dismutase significantly decreased in R group, while it was not affected in P and PR groups. Total protein content significantly decreased in R group in contrast to P group, where a significant increase was observed. In PR group, it was not affected at day 3, but it significantly decreased at day 7. In conclusion, either gamma radiation orprednisolone induced submandibular salivary gland oxidative stress; however, their combined use ameliorated, to some extent, gamma radiation induced oxidative damage.
Egyptian Journal of Radiation Sciences and Applications
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
1110-0303
32
v.
1
no.
2019
13
21
https://ejrsa.journals.ekb.eg/article_28412_1bacccd89cc321a06c63040668f83e73.pdf
dx.doi.org/10.21608/ejrsa.2019.4300.1043
Dose Audits of Patients Undergoing some Common Radiographic Examinations in Selected Nigerian Hospitals
Lateef
Bamidele
Department of Science Laboratory Technology, Osun State College of Technology Esa – Oke, Nigeria
author
Osahon
David
Department of Physics, University of Benin, Benin – City, Nigeria
author
text
article
2019
eng
This study aims at determining radiation doses for patients undergoing six (6) common radio graphic examinations in eight X – ray units in the southern part of Nigeria using computational method. Both public and private – owned hospitals were used for the study. Patient data and technical parameters were collected during the examinations. The meanentrance surface air kerma (ESAK) obtained from all the x-rays units considered ranged from 0.35 to 1.96mGy for Chest PA, 1.01 to 17.55mGy for Abdomen PA; 1.08 to 16.14mGy for pelvis AP,1.12 to 10.06mGy for Pelvis LAT, 0.08 to 3.54mGy for Skull AP/PA; 1.07 to 4.61 for Skull LAT, 1.67 to 12.46mGy for Lumber Spine AP, 1.82 to 14.24mGy for Lumber Spine LAT. Local diagnostic reference levels (LDRLs) were established based on the third quartile of ESAKs values. The established LDRLs were compared with previously established DRLs from other countries. The ESAKs values obtained in this study were found to be within the international established DRLs except in few cases.
Egyptian Journal of Radiation Sciences and Applications
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
1110-0303
32
v.
1
no.
2019
23
29
https://ejrsa.journals.ekb.eg/article_28647_3e3aa3ccc6dbae7d1dffd714daca2a14.pdf
dx.doi.org/10.21608/ejrsa.2019.5325.1053
Ameliorative Effect of Teucrium polium Extract on γ-radiation Toxicity in Brain of Albino Rats
Mostafa
Saif-Elnasr
Health Radiation Research Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Nasr City, Cairo, Egypt
author
Salma
Abdel Fattah
Drug Radiation Research Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Nasr City, Cairo, Egypt
author
Tarik
Mohamed
Phytochemistry Department, National Research Center, Dokki, Giza , Egypt
author
text
article
2019
eng
The impairment to the healthy brain tissue is a crucial factor limiting the application of radiation therapy in patients with nervous system neoplasms. Teucrium polium (TP) L. (family Lamiaceae), a wild-growing flowering plant, has beneficial curative properties. The flavonoids of TP have many valuable biological activities. This study aims atinvestigating theameliorative effect of TP extract on γ-radiation toxicity in brain of albino rats. Thirty adult male albino rats were used. Ionizing irradiation was performed by whole body exposure of rats to 8Gy. TP was administered by intragastric tube in a dose of 200mg/kg body weight. Animals were divided into five groups: Group I (control), Group II (TP), Group III (γ-irradiated), Group IV (TP+γ-irradiated) and Group V (TP+γ-irradiated+TP). The action of TP extract was investigated after one week post irradiation by measuring serum liver and renal functions, and oxidative stress parameters in brain tissue. Moreover, serum S100B and brain-derived neurotrophic factor (BDNF) in brain tissue were measured, in addition tohistopathological examination. TP at a dose of 200mg/kg body weight did not have hepatotoxic or nephrotoxic effects.TP extract significantly ameliorated γ-radiation-induced brain damage by improving radiationinduced oxidative stress. The role of the extract was confirmed by improvement of the levels of S100B in the serum and BDNF in the brain as well as amelioration of histopathological changes induced by radiation. The administration of TP before and after irradiation was more effective than administration only before irradiation. These results revealed that the administration of TP extract might ameliorate γ-radiation-induced brain injury by the attenuation of oxidative stress, the regulation of BDNF and the suppression of S100B.
Egyptian Journal of Radiation Sciences and Applications
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
1110-0303
32
v.
1
no.
2019
31
50
https://ejrsa.journals.ekb.eg/article_29363_1443ec4e7df3ffe2e8b60fb0a13f7fa9.pdf
dx.doi.org/10.21608/ejrsa.2019.9867.1065
The Impact of 950MHz Electromagnetic Radiation on the Brain and Liver of Rats and the Role of Garlic Treatment
Shereen
Shedid
Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
author
Ghada
El-Tawill
Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
author
Fatma
Algeda
Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
author
Neama
El-Fatih
Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, Egypt
author
Noaman
Eltahawy
Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, Egypt
author
text
article
2019
eng
The use of mobile phones, laptops and wireless networks has become essential components of daily life. However, despite they make life easier, they may also cause a number of health problems.This study aims at investigating the changes induced in the liver and brain of male albino rats caused by the exposure to 950MHz and the role of garlic treatment. Male Albino rats were exposed to 950MHz electromagnetic field (power density of 1mW/cm2). Whole body average specific absorption rates (SAR) were 0.238 and 0.372, respectively, for duration of one hour, thrice a week for a period of seven weeks. Garlic extract was administered to the rats at a doseof 500mg/kg body weight, half an hour before each exposure. Animals were sacrificed one day post the last exposure dose. Exposure to 950MHz has triggered oxidative stress in both tissues verified by a significant increase of malondialdehyde (MDA) and nitric oxide (NO) associated with a significant decrease in the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). Exposure to 950MHz has also induced alteration in the xanthine oxidoreductase system (XOR) identified by a significant increase in the activity of xanthine oxidase (XO) concomitant to a significant decrease of xanthine dehydrogenase. The results showed also accumulation of iron (Fe), and cupper (Cu), and decreases in zinc (Zn) and manganese (Mn). Garlic extract treatment has significantly improved these changes. In conclusion, Garlic might attenuate the impact of 950MHz in liver and brain of male rats.
Egyptian Journal of Radiation Sciences and Applications
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
1110-0303
32
v.
1
no.
2019
51
60
https://ejrsa.journals.ekb.eg/article_31561_d52dbf6882859df535be3cb2874ce239.pdf
dx.doi.org/10.21608/ejrsa.2019.7814.1063
Stimulating Effect of Gamma Radiation on some Active Compounds in Eggplant Fruits
Amina
Aly
Natural Products Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
author
Noha
Eliwa
Natural Products Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
author
Mohamed
AbdEl-Megid
Natural Products Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
author
text
article
2019
eng
A FIELD experiment was performed to study the effect of gamma irradiation (0.0, 50 and 100Gy) on growth parameters and bioactive compounds of different parts of eggplant. All growth parameters studied showed an increase when using a dose of 50Gy gamma rays. Also, 50Gy increased total phenolic contents (2.140, 4.039 and 2.965mg/g DW) for pulp, peel and whole fruits, respectively. A dose level at 50Gy increased flavonoid contents (0.835, 4.301 and 3.166mg/g DW) for pulp, peel and whole fruits, respectively. Moreover, tannin contents increased at a dose of 50Gy to (5.853, 7.94 and 7.79mg/g DW) for pulp, peel and whole fruits respectively; the highest contents were detected in peels followed by the whole fruits and the pulps. The DPPH radical scavenging activity of eggplant extracts varies according to the plant part. The antioxidant activity in the whole fruit was less than the peel and pulp while the highest antioxidant activity was in the peel. Otherwise, the amino acid and total soluble protein contents were higher in pulp in all treatments. Phenyl alanine ammonia-lyase (PAL) enzyme and polyphenol oxidase enzyme showed increases in their activities as gamma radiation increased to 50Gy, while increasing irradiation dose level to 100Gy reduces both enzyme activities. FT-IR showed the appearance and disappearance of function group in control and irradiated plants. Meanwhile, the results confirmed the presence of C-H stretching phenol (1020cm-1), alkane C-H blending (1450cm-1) and O-H stretching, lipid at (1450cm-1), and carbohydrate amino acids (3940.39cm-1) in eggplant plants different parts control and irradiated.
Egyptian Journal of Radiation Sciences and Applications
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
1110-0303
32
v.
1
no.
2019
61
73
https://ejrsa.journals.ekb.eg/article_31562_9705bbdfc0218ff94f7b4692b8e73d63.pdf
dx.doi.org/10.21608/ejrsa.2019.10024.1066
Response 5-phosphomevalonate Kinase Enzymes to Low Doses of Gamma Radiation and Its Related to the Production of Terpenoids Indole Alkaloids from Catharanthus rouses
I.
Salama
Natural Products Research Department (Biotechnology Branch), National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt
author
G.
Ali
Natural Products Research Department (Biotechnology Branch), National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt
author
Mohamed
Abd EL-Megid
Natural Products Research Department (Biotechnology Branch), National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt
author
text
article
2019
eng
GAMMA rays effect, on different dose levels on the production of ajmalicine as well as 5-phosphomevalonate kinase enzyme activety, was studied in Catharanthus roseus cell suspension cultures. The seedlings were taken from the young leaf puds and grown on MS solid medium for 7 weeks. Calli individual serially sub cultured by transfing to maintenance medium consisting of Gamborg’s B5 medium. Cell suspensions were exposed to gamma rays after 4 weeks at dose levels of 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5 and 8Gy. Ajmalicine quantities were analyzed on VIS–spectrophotometer; also, the 5-phosphomevalonate kinase enzyme was particularly purified by ultrafiltration and activety analayzed, in addition, native PAGE electrophoresis was determined. 5-phosphomevalonate kinase enzyme bands and ajmalicine accumulation quantities were changed after the exposure to low doses from 1 to 6Gy, but the higher doses were lethal. The appropriated doses to activety of 5-phosphomevalonate kinase enzyme and Ajmalicine production were from 1 to 5Gy. Other dose levels (5.5 and 6Gy) did not affect on the 5-phosphomevalonate kinase enzyme activety and Ajmalicine production.
Egyptian Journal of Radiation Sciences and Applications
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
1110-0303
32
v.
1
no.
2019
75
85
https://ejrsa.journals.ekb.eg/article_31619_4634d7e572565aeda96e2f84c4d23cdd.pdf
dx.doi.org/10.21608/ejrsa.2019.7148.1061
Alteration of Cell Mediated Immunity in Vitiligo Patients Using Narrow Band UVB as a Treatment
Noha
Ibrahim
Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
author
Nashwa
Radwan
Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
author
Lailaa
Rashed
Biochemistry Department, Cairo University, Giza, Egypt
author
text
article
2019
eng
VITILIGO is considered an autoimmune depigmenting disease. There were many evidences suggested the role of T cell mediated immunity and cytokines in the pathogenesis of the disease. The study included 20 active vitiligo patients (group 1), 20 treated patients using narrow band ultraviolet radiation B (NBUVB) (group 2), and 20 healthy control of matching age and sex (group 3). IL- 17, IL-10, TGF- Bı levels in skin tissue were measured in the three groups using ELISA technique. The results showed a significant increase in IL- 17 and TGF-Bı while there was a significant decrease in IL-10 in active vitiligo patients compared to the control (P= 0.000). Following treatment using NB-UVB, the results showed a significant decrease in the level of both IL- 17 and TGF-Bı while there was a significant increase in IL-10 (P= 0.000) compared to active vitiligo group. These statistically significant results suggest the cell mediated immune role in the disease and successful treatment by the narrow band UVB that altered the cytokines toxic effect.
Egyptian Journal of Radiation Sciences and Applications
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
1110-0303
32
v.
1
no.
2019
87
94
https://ejrsa.journals.ekb.eg/article_34331_cce15d2f1e0e09e90e9d0b7b6a4a35f2.pdf
dx.doi.org/10.21608/ejrsa.2019.11066.1067
Preparation of 186Re-Cefixime as a Potential Diagnostic and Therapeutic Agent for Bacterial Infection
Hanan
Talaat
Labeled Compounds Department, Hot Labs. Center, Atomic Energy Authority (AEA), Cairo, Egypt
author
Mohamed
Aydia
Radioactive Isotopes and Generators Department, Hot Labs. Center, Atomic Energy Authority (AEA), Cairo, Egypt
author
Ismail
Ibrahim
Labeled Compounds Department, Hot Labs. Center, Atomic Energy Authority (AEA), Cairo, Egypt
author
Hassan
El-Said
Radioactive Isotopes and Generators Department, Hot Labs. Center, Atomic Energy Authority (AEA), Cairo, Egypt
author
Khaled
El-Azony
Radioactive Isotopes and Generators Department, Hot Labs. Center, Atomic Energy Authority (AEA), Cairo, Egypt
author
text
article
2019
eng
SEVERAL factors that influence the preparation of 186Re-Cefixime such as the amount of Cefixime and stannous chloride, pH, reaction time and reaction temperature were studied to optimize the labeling conditions to obtain the highest radiochemical yield of 186Re-Cefixime. The radiochemical purity of Rhenium-186 was determined by paper chromatography, while the radiochemical yield and purity of 186Re-Cefixime were determined by electrophoresis and high-performance liquid chromatography (HPLC). The maximum radiochemical yield of 186Re-Cefixime was obtained (96±2.8%) using 2mg Cefixime, 0.3mg carrier added 186Re and 0.5mg stannous chloride at pH 5.5, within 30min at room temperature. The bio-distribution was carried out on three types of mice (normal, sterile infected and bacterial infected). The results show that 186Re-Cefixime is more concentrated in the bacterially infected muscle (septic inflammation) than in the sterile infected muscle (aseptic inflammation). Therefore, 186Re-Cefixime could be used to differentiate between septic and aseptic inflammation.
Egyptian Journal of Radiation Sciences and Applications
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
1110-0303
32
v.
1
no.
2019
95
103
https://ejrsa.journals.ekb.eg/article_38224_0ee087e23936d080f1cab85fb427af8e.pdf
dx.doi.org/10.21608/ejrsa.2019.6992.1059
Application of Metakaolin-slag Binders to Solidify Rad-waste Glass Sharps: Leaching of 137Cs and 152+154Eu Radionuclides
M.
El-Naggar
Hot Laboratories Center, Atomic Energy Authority (AEA), Cairo, Egypt
author
M.
Ali
Hot Laboratories Center, Atomic Energy Authority (AEA), Cairo, Egypt
author
A.
El-Sadek
Hot Laboratories Center, Atomic Energy Authority (AEA), Cairo, Egypt
author
H.
El-Said
Hot Laboratories Center, Atomic Energy Authority (AEA), Cairo, Egypt
author
text
article
2019
eng
METAKAOLIN (MK) and blast furnace slag (BFS) were utilized to design alkali activated binders suitable for solidification of waste glass (WG sharps contaminated with 137Cs and 152+154Eu radionuclides). Two forms of WG, powdered and fused, were utilized. Effects of WG forms and BFS addition on the ability of formulations to withstand compression were preliminary studied. When cold powdered WG was solidified the corresponding compressive strength (MPa) values were decreased by increasing the loading percentages. While fused forms were positively affected MPa values till 10.0% loading. Positive relations between BFS addition and MPa values were also detected. Leaching of 137Cs and 152+154Eu from MK-based formulations was followed as affected by WG forms, BFS addition and early age (2hr) curing temperatures. Both studied radionuclides were cumulatively leached in larger fractions from solidified powdered WG than from fused one. The obtained results reflected the ability of the designed formulations to retain 137Cs in greater extents than 152+154Eu. The lowest diffusion coefficient values (2.01×10-11 and 3.92×10-10cm2/s) were recorded when fused forms of WG containing 137Cs and 152+154Eu, respectively, were solidified into slag seeded MK-based formulations, early aged cured at 40°C. Slope values of log(CLF) vs. log(time) plots were < 0.35 indicating that the controlling leaching mechanisms for all studied leaching systems were predicted to be wash-off.
Egyptian Journal of Radiation Sciences and Applications
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
1110-0303
32
v.
1
no.
2019
105
115
https://ejrsa.journals.ekb.eg/article_40315_1cbf1f51926a7146342c08e49eae443e.pdf
dx.doi.org/10.21608/ejrsa.2019.13301.1072
Reflex of Oleandrin Production and Molecular Changes on the Nerium oleander Cell Suspension Culture under UV-A Radiation Stress Effect
Islam
Salama
Natural Products Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt
author
Mohamed
Abd el-Megid
Natural Products Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt
author
text
article
2019
eng
THE AIM of this study is to determine the oleandrin concentrations in the Nerium oleander cell suspension culture after being exposed to UV-A (365nm), at different time intervals (0 controls, 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50min). The results showed that the maximum time limits for carrying N. oleander cells suspension culture to exposure of UV-A is 30min.The present study also aims at investigating changes of the protein bands, peroxidase isozyme and oleandrin concentration at the same condition. Oleandrin was separated and quantified by HPLC. The investigation of protein changes was conducted using SDS PAGE electrophoresis techniques. The results revealed that the changes of the protein bands depend on different time intervals of exposure to A-UV radiation. Thus, the changes of the oleandrin production were consequent to gene expression changes. Peroxidase isozyme analysis, showed that exposure to UV-A for the 5, 15 and 20min had a clear impact on the peroxidase isozyme, but the greatest impact was in the sample exposed to UV-A for a period of 15 minutes (0.093±0.01) μg/ml, the same sample showed the highest concentration of oleandrin compared to the control (0.022±0.001) μg/ml. However, the other samples were less affected by the exposure to UV-A.
Egyptian Journal of Radiation Sciences and Applications
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
1110-0303
32
v.
1
no.
2019
117
124
https://ejrsa.journals.ekb.eg/article_42603_f8a738ffaf6c27f5fc9835e0b50dc2d3.pdf
dx.doi.org/10.21608/ejrsa.2019.7166.1062