Genetic Variant Detected by RAPD-PCR and ISSR in Catharanthus roseus (L.) Cells Exposed to Low Doses of Gamma Rays

Document Type : Original Article

Abstract

 CATHARANTHUS ROSEUS (L.) (C. roseus) 10 samples, genetically different of irradiated and control cell suspension culture were detected by both random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and inter simple sequence repeat (ISSR). The RAPD-PCR and ISSR-PCR profiles were used for building phenetic trees by using Totallab Quant software, showing similarity in the topology of the trees. Both dendograms presented three major clusters that 10 samples irradiated and control, according to genetic similarity.
The control and the irradiated samples at 2.5 and 4Gy, which are highly in the similarity index recorded as 0.101, while the lowest similarity index recorded was 0.058, which was observed between 3.5 and 4.5Gy.
A dendrogram RAPD-PCR for the genetic relationships among the 10 samples irradiated and control of
 C. roseus the cell suspension culture taxa was carried out. The 10 samples irradiated and control from C. roseus cell suspension culture taxa were separated into three clusters; cluster one included 1, 1.5, 2 & 3Gy, while the cluster two included control, 2, 2.5, 3.5, 4 & 4.5Gy. The cluster three included 4.5 & 5Gy.
The best doses of gamma rays were from 0.5 to 5Gy in order to
 C. roseus cells genome manipulation and induced mutations. The genome modification was the stimulation of the gene expression changes in order to changes of physiological cell and production of cell lines. The aim of the production cell lines to elicit cells enjoys the attributes of high productivity, secondary products, which are used in widely used in the pharmaceutical industry.

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