Preparation and Purification of HBsAg from HBsAg-Positive Human Blood

Farag, Marwa Hany; Shouman, Sahar A.H.; El-Bayoumy, Ahmed S.A.; Abdel Mohty, Ahmed A.; Mehany, Nagy L.; Gado, Marwa M.

doi:10.21608/ejrsa.2022.128612.1133

Preparation and Purification of HBsAg from HBsAg-Positive Human Blood

Document Type : Original Article

Authors

¹ Labeled Compounds Department, Hot Laboratories Center, Atomic Energy Authority, Cairo, Egypt, [2] Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt,

² Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt

³ Labeled Compounds Department, Hot Laboratories Center, Atomic Energy Authority, Cairo, Egypt

⁴ Radioisotopes and Generator Department, Hot Laboratories Center, Atomic Energy Authority, Cairo, Egypt

10.21608/ejrsa.2022.128612.1133

Abstract

HEPATITIS B virus (HBV) is one of the most serious world health problems as it is highly distributed and can result in very dangerous diseases including liver cirrhosis, liver failure or even liver carcinomas. Thus, it is very important to early diagnose it. One of the most important tools to diagnose HBV is to measure the presence of hepatitis B surface antigen (HBsAg) in patient blood. A special kit is the one of the most potent tools for this purpose, in which HBsAg is essential component. Hence, in the present study the authors prepared and purified HBsAg which can be later used as an antigen, to prepare specific antibodies and for other applications.

Keywords