eng
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Radiation Sciences and Applications
1110-0303
2314-8527
2018-12-01
31
2
89
95
10.21608/ejrsa.2017.1816.1022
4713
Original Article
Activation Role of Low Dose Ionizing Radiation on Human Lymphocytes Response
Sherien Montaser
sherien_morsy@yahoo.com
1
Gehan Roshdy Abdel Hamid
gih_ro@yahoo.com
2
Ahmad Rashad Aboghadeer
ahmedaboghadeer@hotmail.com
3
Radiation Biology Department, National Centre for Radiation Research and Technology (NCRRT), Atomic Energy Authority, P. O. Box: 29 Nasr City, Egypt
Radiation Biology Department, National Centre for Radiation Research and Technology (NCRRT), Atomic Energy Authority, P. O. Box: 29 Nasr City, Egypt
Radiation Biology Department, National Centre for Radiation Research and Technology (NCRRT), Atomic Energy Authority, P. O. Box: 29 Nasr City, Egypt
LOW dose ionizing radiation can imitate the role of deteriorate antigen or attenuated microbes as it activates the first defence line of immune response, the step which when lost, cancer cells can find suitable environmental condition for growth propagation. Human whole blood samples were irradiated in vitro at doses (0.25, 0.5, 0.75 and 1Gy) of ionizing radiation and then kept at 37oC for 1h before measuring glucose concentrations which reflect the maximum activation of immune cells. Then, immunological parameters such as interlukine-1β (IL-1β), interlukine-2 (IL-2), transforming growth factor beta (1) (TGF-β1) were investigated. Also, inflammatory mediators as intracellular adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1) and tyrosine kinase activity were determined. Low doses of ionizing radiation induced slight activation of the immune cell cytokines (IL-1β and TGF-β1), inflammatory mediators (ICAM, VCAM) and tyrosine kinase activity at doses (0.25, 0.5Gy) reaching to significant levels at 0.75Gy and 1Gy. While, IL-2 and glucose levels were significantly decrease.
https://ejrsa.journals.ekb.eg/article_4713_96b37c66ddc9cd838e7095bd2648706a.pdf
Gmama-irradiation
IL-1β
IL-2
TGF-β
ICAM and VCAM
eng
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Radiation Sciences and Applications
1110-0303
2314-8527
2018-12-01
31
2
97
109
10.21608/ejrsa.2018.2092.1030
5862
Original Article
Combating Bacterial Adhesion and Protein Deposition on Cosmetic Contact Lenses Using Zinc Oxide Nanoparticles
Eman Araby
emansk2@gmail.com
1
Hanan Elbastawisy
hanan.elbastawisy2015@gmail.com
2
Seham El-Tablawy
seham_eltablawy@yahoo.com
3
National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority
Drug Radiation Research Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), P.O. Box 29,Nasr City, Cairo, Egypt.
Drug Radiation Research Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), P.O. Box 29,Nasr City, Cairo, Egypt.
CONTACT lenses (CLs) are frequently used for medical or cosmetic purposes. Readily available cosmetic contact lenses (CCLs) sold at night market and lack of awareness, represent the main reasons for microbial keratitis (MK) and consequently losing sight. MK arises from bacterial contamination of lenses, lens casing and solutions. Improper handling and unhygienic use of the CLs allow pathogens to adhere and produce biofilm on its surface. This study aims to evaluate the adherence ability of pathogenic bacterial strains isolated from worn CCLs and the potentiality of zinc oxide nanoparticles (ZnO NPs) on their colonization in absence and presence of two types of deposited proteins. The results revealed that all the isolated strains had the ability to produce strong biofilm. ZnONPs highly affected the biofilm formation ability of the tested strains and significantly decreased their adherence on the tested proteins coated lenses. Using scanning field emission electron microscopy; extensive colonization covering CCLs surface of control and lysozyme coated lenses was observed. While micrographs illustrated that ZnONPs are effective in restraining bacterial colonization with deformed cells on the surface of lysozyme coated lenses. In addition, the data exhibited that 75% of the tested packaging solutions were highly contaminated with bacteria, so they were subjected to increasing doses of gamma- radiation. The results proved that 6.0kGy was sufficient to eliminate bacterial bio burden of the tested solutions. It may be hoped that the present study will encourage efforts towards the development of novel antibacterial agents against microbial infection of CCLs.
https://ejrsa.journals.ekb.eg/article_5862_e04ec172263c8c91850547c75a216062.pdf
Cosmetic contact lenses
Protein coated lenses
Zinc Oxide Nanoparticles
Bacterial adhesion
Gamma- radiation
eng
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Radiation Sciences and Applications
1110-0303
2314-8527
2018-12-01
31
2
111
124
10.21608/ejrsa.2018.2008.1032
5860
Original Article
Panax ginseng Extract Modulates Cerebral Damage Caused by Gamma Irradiation in Male Albino Rats
Shereen Elkiki
shereenelkiki@yahoo.com
1
Shereen Galal
shereen.galal@yahoo.com
2
Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), PO Box 29, Nasr City, Cairo, Egypt
Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), PO Box 29, Nasr City, Cairo, Egypt
THIS STUDY is designed to investigate the protective effect of aqueous Panax ginseng extract against acute brain damage induced by gamma irradiation in rats. Animals were sacrificed 24h post-whole body gamma irradiation (6Gy). The results revealed oxidative injury denoted by a significant increase in the levels of malondialdehyde (MDA) associated to a significant decrease in the level of reduced glutathione (GSH), and superoxide dismutase (SOD) and catalase (CAT) activities in brain tissues. The administration of Panax ginseng at a dose of 100mg/kg body weight for 7 consecutive days before irradiation has significantly attenuated the severity of oxidative stress. Furthermore, Panax ginseng has significantly improved the decrease recorded in the level of the neurotransmitters serotonin (5-HT), dopamine (DA), norepinephrine (NE) and acetylcholine (Ach) induced by gamma irradiation in brain tissue. The oral administration of ginseng extract prior to irradiation has attenuated also a significant decrease in serum asymmetric dimethyl arginine (ADMA) as well as a significant increase in serum nitric oxide (NO) compared with the irradiated group. The histopathological and immunohistochemical examination substantiate the aforementioned amelioration in the biochemical parameters. In conclusion, the Panax ginseng extract might alleviate the oxidative brain damage and the alteration of neurotransmitters induced by gamma-irradiation in rats.
https://ejrsa.journals.ekb.eg/article_5860_89bdab2847a2fa5c745ec61ccb757ab6.pdf
Panax ginseng
Gamma Irradiation
Oxidative Stress
neurotransmitters
eng
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Radiation Sciences and Applications
1110-0303
2314-8527
2018-12-01
31
2
125
136
10.21608/ejrsa.2018.2286.1035
16138
Original Article
A Physiological and Genetical Study for the Effect of Gamma Irradiation on Moringa olefiera Lam.
Hanan Mansour
hanan82mm@yahoo.com
1
Nahla Hamideldin
n.hamideldin@yahoo.com
2
Fatthy Abdel-Tawab
fatthy38@yahoo.com
3
Eman Fahmy
emanfahmy@yahoo.com
4
Hoda El Demerdash
hodaeldemerdach@yahoo.com
5
Mohamed Amar
mhasnna@yahoo.com
6
Natural Product Research Dept., National Center for Radiation Research and Technology, Atomic Energy Authority (AEA), Cairo, Egypt
Natural Product Research Dept., National Center for Radiation Research and Technology, Atomic Energy Authority (AEA), Cairo, Egypt
Genetics Department, Faculty of Agriculture., Ain Shams University, Cairo, Egypt
Genetics Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
Natural Product Research Dept., National Center for Radiation Research and Technology, Atomic Energy Authority (AEA), Cairo, Egypt
Egyptian Deserts Gene Bank, Desert Research Center, Cairo, Egypt
SEEDS of Moringa oleifera plant were exposed to different gamma irradiation doses; 0, 20, 40 and 60Gy. Effects of gamma irradiation on a number of vegetative, physiological and molecular characters were studied. The vegetative characters were studied for two seasons, the highest values for the studied characters were often observed at the dose of 40Gy (8 characters) then at the dose of 60Gy (4 characters). The physiological characters studied were total phenolic compounds contents, amino acid contents and antioxidant enzymes activity. Total phenols contents showed a significant increase in all used doses compared with the control. Amino acids content was affected widely by gamma doses, especially with 20 and 40Gy.The effect of gamma irradiation on the activity of five antioxidant enzymes (catalase, polyphenol oxidase, peroxidase, superoxide dismutase and phenylalanine ammonia lyase) was studied. Isozymes electrophoresis was carried out for two isozymes (peroxidase and polyphenol oxidase). New bands were detected which can be used as markers for gamma irradiation.
https://ejrsa.journals.ekb.eg/article_16138_6f2cb160386680a2f4deeaa265d924ea.pdf
Moringa oleifera
Gamma rays
antioxidants
isozymes
eng
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Radiation Sciences and Applications
1110-0303
2314-8527
2018-12-01
31
2
137
142
10.21608/ejrsa.2018.3021.1040
14032
Original Article
ESR Studies on Characteristic Physio-chemical Effects of Some Irradiated Antibiotics
Saleh Abdou
salehabdou03@yahoo.com
1
S. Ebraheem
seif_dose@yahoo.com
2
Radiation Physics Department, NCRRT, Atomic Energy Authority (AEA), Cairo, Egypt
Radiation Protection Department, NCRRT, Atomic Energy Authority (AEA), Cairo, Egypt
MOST of the characteristic chemical effects of the irradiated solid drugs are not accurately detected by the conventional tests such as ultra violet spectro-photometry and melting point technique. Sterilization using gamma irradiation is a desirable alternative for the thermo-sensitive drugs. Antibiotics samples were -irradiated at room temperature with doses ranging from 2 kGy up to 25 kGy. Electron Spine Resonance (ESR) measurements were applied on four types of antibiotics; Amoxycillin, Ampicillin, Dalacin and Spiramycin (AMX, AMP, DAL and SPI). ESR results exhibited the impact of irradiation and storage parameters on the nature and concentration of the trapped radicals. The radical concentration is directly proportional to the ESR peak height signal also depending on the absorbed dose delivered to the antibiotic samples. The obtained information about the irradiated radiolytic products was found to verify the radical mechanisms. This information may be used to detect an irradiation treatment on such pharmaceuticals.
https://ejrsa.journals.ekb.eg/article_14032_5b80ae3f6a1c3e3114dc379148d03995.pdf
Drug sterilization
ESR
Ionizing radiation
Gamma Irradiation
eng
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Radiation Sciences and Applications
1110-0303
2314-8527
2018-12-01
31
2
143
153
10.21608/ejrsa.2018.3184.1042
16167
Original Article
Impacts of Irradiated Distillers Dried Grains with Soluble (DDGS) as Dietary Supplement on Physiological and Biochemical Aspects of Growing Rabbits
A. Abdul Azeem
alyncrrt@yahoo.com
1
U. Abdel-Monem
ormamohamed-2010@yahoo.com
2
Ashraf Mounir
ash1810@hotmail.com
3
Food Irradiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, Egypt
Department of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig, Egypt
Food Irradiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, Egypt
D istillers dried grains with soluble (DDGS) are a co-product of ethanol production from starch cereals (mainly corn). Dietary fiber is the sum of carbohydrates and lignin that are resistant to digestion by the small intestine enzymes. DDGS contains approximately 3
times dietary fiber more than corn. Dietary fiber in DDGS consists mainly of insoluble dietary fiber. The present study was conducted to evaluate the effect of gamma (γ) irradiation on crude fiber, soluble fiber and non-soluble fiber of irradiated corn DDGs at different doses 0, 10, 20 and 30kGy, and to evaluate the effect of feeding irradiated DDGs for rabbits and their effects on rabbit’s performance (body weight, gain weight, feed intake, feed conversion, water intake, rectum temperature and respiration rate), apparent digestibility (dry matter, crude protein, crude fiber and ether extracts), carcass traits (carcass weight, dressing (%) and prime cuts (%) and blood biochemistry (AST, ALT , total protein, albumin, globulin, creatinine, total lipids, total cholesterol, and blood urea). Sixty New Zealand White weaned male rabbits of 35 days age were randomly divided to 4 treatment groups of approximately equal average weight (706g) with 15
animals each. The first group was fed the basal diet supplemented with 10% DDGS (non irradiated) (control), 2 nd, 3rd and 4th groups were fed the control diet supplemented with 10% irradiated DDGS at 10, 20 and 30kGy, respectively. The obtained results revealed a decrease in crude fiber and increases in soluble fiber and insoluble fiber. The increases were linearly correlated with the increasing irradiation dose. The supplemented irradiated 10% DDGS up to 30kGy in the diet of growing rabbits resulted in a significant improvement of body weight, gain weight, feed conversion, the apparent digestibility of dry matter, crude protein, crude fiber, carcass traits (carcass weight, dressing (%) and prime cuts(%)), and the improvement was
parallel with increasing the radiation dose. While, feed intake, water intake, rectal temperature, respiration rate, ether extracts digestibility, AST, ALT , total protein, albumin, globulin, total lipids, total cholesterol, creatinine and blood urea were not altered by the treatments. Generally, it can be concluded that radiation processing with γ-rays improved the soluble and insoluble fiber and the digestibility of DDGS, and that DDGS supplemented diets fed to growing rabbits improved rabbits' growth performance without any deleterious effect on physiological and biochemical attributes.
https://ejrsa.journals.ekb.eg/article_16167_4e823b40ad1614e0e8a6de0f9231f719.pdf
growing rabbits
DDGS
diet
digestibility
Biochemical attributes
gamma radiation
eng
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Radiation Sciences and Applications
1110-0303
2314-8527
2018-12-01
31
2
155
166
10.21608/ejrsa.2018.4491.1046
14034
Original Article
Concerted Effects of L-carnitine and Vitamin E on Cardiopulmonary Apoptosis Induced by Gamma Irradiation in Rats
Heba Mansour
hebahosny@hotmail.com
1
Shereen Galal
shereen.galal@yahoo.com
2
Shereen El Kiki
shereenelkiki@yahoo.com
3
Health Radiation Research Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
Health Radiation Research Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
Health Radiation Research Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
Pulmonary fibrosis and cardiotoxicity are a common and dose-limiting side-effect of ionizing radiation used to treat cancers of the thoracic region. This study aims at investigating the role of Bcl-2, Bax protein and caspase-3 in the pathogenesis of tissue damage induced by gamma irradiation (IRR) and the protective potential role of vitamin E (Vit E) and/or L-carnitine (L-car) against γ-irradiation induced oxidative injury. Irradiation was performed as a whole body γ-irradiation (5Gy). Immnuohistochemistry was used to estimate Bcl-2 expression. The level of Bax and caspase 3 activity were determined using enzyme-linked immunosorbent assay. C-reactive protein was determined using immunoturbidimetric assay. Results revealed that γ-irradiation (5Gy) induced apoptosis via the mitochondrial pathway by downregulating Bcl-2 expression, upregulating Bax protein and activating caspase-3. Administration of Vit E (50mg/kg) and/or L-car (300mg/kg) prior to gamma-irradiation decreased apoptosis through changing the expression of Bcl-2, Bax and caspase-3 activity. Exposure to γ-irradiation increased triglyceride, cholesterol, low density lipoprotein-cholesterol (LDL-C), C-reactive protein (CRP) and potassium levels, creatine kinase (CK-MB) and lactate dehydrogenase (LDH) activities and decreased high density lipoprotein-cholesterol (HDL-C) levels. The altered lipid profile, CRP and potassium levels, CK-MB and LDH activities induced by exposure to γ-irradiation were significantly renovated in Vit E and/or L-car pretreated γ-irradiated rats. In conclusion, Vit E and/or L-car might ameliorate γ-irradiation induced apoptosis via modulation of Bcl-2, Bax and caspase-3.
https://ejrsa.journals.ekb.eg/article_14034_3e2adbc3c80c667f077eb52adaa0d912.pdf
Gamma Irradiation
L-carnitine
Vitamin E
Caspase-3
Bcl-2
Bax
eng
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Radiation Sciences and Applications
1110-0303
2314-8527
2018-12-01
31
2
167
175
10.21608/ejrsa.2018.4659.1047
23603
Original Article
Immunomodulatory Effect of Irradiated β-glucan in Diethylnitrosamine Induced Renal Toxicity
Gehan Moawed
gih_ro@yahoo.com
1
Lobna Abdel-Aziz
2
Lobna Anees
3
Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
β-glucans are one of the most abundant forms of polysaccharides known as biological response modifiers which influence host’s biological response and stimulate immune system. Accordingly, this study is carried out to evaluate the effect of gamma irradiated β-glucan (Iβ-glucan) extracted from mushroom in modulating diethylnitrosamine (DEN)-induced immune response and mitochondrial dysfunction in renal toxicity. Rats were divided into 4 groups. Group I: control group, group II: animals received the DEN (20mg/kg) for 6 weeks, group III: was gavaged Iβ-glucan (65mg/kg b. wt.) for 6 weeks, group IV: received as group II and then treated as group III. Exposure to DEN induced a change in the levels of cytokines (IFN-γ, TNF-α, IL-6) and nephrotoxicity, which was proved by significant elevated levels of creatinine and urea along with upregulation of mRNA cytochrome P450 (2E1) and downregulation in mitochondrial enzymes complex I and II. Treatment with Iβ-glucans post DEN-exposure significantly improved the disturbances in all the ++ tested parameters. It could be concluded that Iβ-glucan treatment exerts a potential biological effect on both mitochondrial and immune system dysfunction caused by the DEN- induced renal toxicity in rats. It is considered for further trials.
https://ejrsa.journals.ekb.eg/article_23603_762d9044b5819cd8f37bffff72656cb9.pdf
Iβ-glucan
DEN
Cytokines
Cytochrome P450 (2E1)
Mitochondrial complexes
eng
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Radiation Sciences and Applications
1110-0303
2314-8527
2018-12-01
31
2
177
184
10.21608/ejrsa.2018.4820.1048
25260
Original Article
Histological and Immunohistochemical Evaluation for the Effect of Pilocarpine and Quercetin on Gamma-irradiated Parotid Salivary Glands
Said Hani
hani.said@yahoo.com
1
Abd Elnasser Abd Elmawla
nasserawad116@gmail.com
2
Salwa Ahmed
salwaa_fared@yahoo.com
3
Ashraf Emran
ashrafomran53@yahoo.com
4
Oral Biology Department, Faculty of Dental Medicine for Boys, Al-Azhar University, Cairo, Egypt
Oral Biology Department, Faculty of Dental Medicine for Boys, Al-Azhar University, Cairo, Egypt
Health Radiation Research Department, National Centre for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
Oral Biology Department, Faculty of Dental Medicine for Boys, Al-Azhar University, Cairo, Egypt
Radiotherapy of patients with head and neck tumors usually causes damage to the salivary glands since these are most frequently included in the field of irradiation. This study aims at investigating the possible protective effect of pilocarpine or quercetin against radiation induced parotid gland damage. Forty-five adult male rats were divided randomly into three main groups as follows: Radiation group where the rats were exposed to a single whole body 6Gy γ-irradiation; pilocarpine- and quercetin-radiation groups where the rats injected intrapretoneally by a single dose of pilocarpine (0.2mg/kg) or quercetin (1.25g/kg), respectively, 30min later exposed to a single whole body 6Gy γ-irradiation. The parotid glands were stained with hematoxylin and eosin and immunohistochemical reaction for vascular endothelial growth factor A (VEGF-A). Irradiated glands revealed massive acinar atrophy, degeneration and cytoplasmic vacuolization while, the ducts showed cytoplasmic vacuolizations with loss of regular cell architecture. Pilocarpine or quercetin treatment before radiation exposure offered some protection effect manifested as reduced acinaratrophy, degeneration and vacuolization. The ducts showed mild cellular vacuolizations. Regarding VEGF-A immunoreactivity, gamma irradiated parotid gland showed intense expression and significantly higher area that has persisted up to sixteen weeks. Pilocarpine or quercetin treatment was associated with lesser degree and significantly lower area percent of VEGF-A expressionas compared to the radiation group. Pilocarpine or quercetin has some improvement for the injurious effects of radiation on salivary glands so, they can be used as radioprotective agents before radiotherapy schedules to decrease radiation side effects on salivary glands.
https://ejrsa.journals.ekb.eg/article_25260_8db2b855062a435cc8eaafb87723c9a1.pdf
gamma-irradiation
Pilocarpine
Quercetin
Parotid
vascular endothelial growth factor
eng
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Radiation Sciences and Applications
1110-0303
2314-8527
2018-12-01
31
2
185
193
10.21608/ejrsa.2018.5656.1054
23606
Original Article
Expression of dnak, groES and cps Genes in Irradiated Klebsiella pneumoniae Strains Isolated from UTI Egyptian Patients
Hanady Nada
hanadynada@hotmail.com
1
Sanaa Hagag
sanaahagag@hotmail.com
2
Seham El-Tablawy
seham_eltablawy@yahoo.com
3
Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
Radiology Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
T his investigati on presents a brief study for monitoring the correlation between the expression of heat-shock protein (HSP) genes; dnak, and groES as well as the capsular polysaccharide (caps) gene and antimicrobial susceptibility of extensively drug resistant (XDR) Klebsiella pneumoniae in response to a low dose of Gamma (γ) radiation. The XDR K. pneumoniae strains, that were obtained from (UTI) Egyptian patients were identified using the API 20E strip automated system. Their antimicrobial susceptibilities were monitored using standard disc diffusion method in parallel with their minimum inhibition concentrations (MICs) towards standard antibiotics using a micro-titer method, before and after exposure to γ-radiation at a dose of 24.4 Gray (Gy). The expression of dnak, groES, and cps genes was detected by qRT-PCR under the same conditions. The results demonstrate increasing antibiotic resistance after exposure to γ-radiation. Also, a doubling in the MIC of the tested strains towards CAZ(ceftazidime) was observed. These results were confirmed by an elevation in the expression of dnak, groES, and cps genes in response to γ-radiation. These preliminary results need more confirmaions, because they assume difficulties in curing immunocompromised patients infected with XDR K. pneumoniae by the currently available antibiotics.
https://ejrsa.journals.ekb.eg/article_23606_9f05e9d1c2d96c8089f5344be7b0ac2b.pdf
Pathogenic bacteria
Virulence factor
Antibiotic resistance
Ionizing radiation
Heat-shock protein genes
eng
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Radiation Sciences and Applications
1110-0303
2314-8527
2018-12-01
31
2
195
204
10.21608/ejrsa.2018.6005.1055
23605
Original Article
Biochemical Impacts of Treating Diabetic Rats with Irradiated Cinnamon
Mohamed Abd el-Megid
hassan.m3231@yahoo.com
1
Ashraf Mounir
ash1810@hotmail.com
2
A. Abdul Azeem
alyncrrt@yahoo.com
3
A. el-Shahat
amrnagh.4a@yahoo.com
4
Natural Products Department, National Centre for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, Egypt
Food Irradiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
Food irradiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
Food irradiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
T HE OBJECTIVES of the present study are to evaluate the effect of γ-radiation on total phenolic contents of cinnamon dried powder (CDP) and also to investigate the role of raw and γ-irradiated cinnamon extract (CE) in ameliorating biological disturbances in alloxan-induced diabetic rats. The results obtained show that γ-irradiation (10kGy) increases the total phenolic contents of CDP by percent change 6.39%. The results revealed also that alloxan administration to rats (150mg/kg B.WT) showed a significant increase in the levels of serum glucose, urea, creatinine, uric acidconcomitant with a significant decrease in the levels of insulin, testosterone (T), serum total thyroid hormones triiodothyronin (T3) and thyroxine (T4). Furtherer more, alloxan induced asignificant elevation in lipid peroxidation and a reduction in glutathione content and antioxidant enzyme activities in the renal and testicular tissues of diabetic rats. Treatment of diabetic rats with either raw or γ-irradiated CE has significantly ameliorated the alteration in the antioxidant/oxidant status and improved hyperglycemia, renal, and endocrine abnormalities. In conclusion, the study indicated that cinnamon, in addition to being hypoglycaemic, is effective in reducing oxidative stress caused by alloxan-induceddiabetes and the γ-irradiation of CPE increased its hygienic quality and significantly its total phenolic content without a significant loss in its quality attributes.
https://ejrsa.journals.ekb.eg/article_23605_1ca43c8849c355f9a2c7527811d4f40d.pdf
Cinnamon
antioxidants
diabetes
alloxan
Oxidative Stress
gamma radiation
eng
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Radiation Sciences and Applications
1110-0303
2314-8527
2018-12-01
31
2
205
213
10.21608/ejrsa.2018.6257.1056
23607
Original Article
A Comparative Analysis of VEGF Expression in Systemic Lupus Erythematosus with and without Nephritis
Dina Elessawi
dinaelessawi@hotmail.com
1
Nashwa Radwan
radwanashwa@yahoo.com
2
Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
T his work aims at evaluating the level of Vascular Endothelial Growth Factor (VEGF) in systemic lupus erythematosus (SLE) patients and studying its association with diseasing the activity and lupus nephritis(LN). The study was carried out on 40 SLE, 40 LN patients and 20 matched controls. Plasma levels of (VEGF) expression were measured using ELISA technique. Forty SLE and 40 LN patients with a mean age of (31.62±8.27) years while 20 healthy controls were (30.28±9.02) years and the SLE Disease Activity Index (SLEDAI) was (9.1±5.5). In the SLE patients, anti-nuclear antibodies (ANA) was positive in 47.5% and the anti-double stranded deoxyribonucleic acid (Anti-ds DNA) was positive in 52.5% of the patients while in LN (ANA) was positive in 47.5% of patients. Anti-ds DNA was positive in 62.5%. VEGA expression level was significantly increased in LN with mean (470.1±369.4U/ml) compared to the SLE patients with mean (183.54±95.03) with a p value (P=0.0001). A comparative analysis between immunological and biochemical data of the SLE patients and the lupus nephritis patients show an increase in the mean levels of ESR and creatinine in lupus nephritis patients compared to the SLE patients (P<0.005). VEGF level in the SLE patients is not associated with skin ulcer, Oral ulcers, malar rash, arthritis, proteinuria and anemia (P<0.05) while in the LN patients, it statistically significant increased with skin ulcer, Oral ulcers, malar rash, arthritis and anemia (P<0.05). It could be concluded that VEGF level is higher in the lupus nephritis group compared to the SLE without nephritis and control groups.
https://ejrsa.journals.ekb.eg/article_23607_96d0154231cb5fbc53f57eea02db8028.pdf
VEGF
SLE
Lupus nephritis
eng
The National Centre for Information and Documentation (NIDOC), The Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Radiation Sciences and Applications
1110-0303
2314-8527
2018-12-01
31
2
215
224
10.21608/ejrsa.2019.3684.1057
25462
Original Article
Radiographic and Histopathologic Evaluation of L-Carnitine and Vitamin E Efficacy on Irradiated Jaw Bones
Amira Sayed
emmy_dr2000@hotmail.com
1
Osama El-Shall
oelshall@hotmail.com
2
Dina El Husseiny
drdinahusseiny@yahoo.com
3
Mai Attia
mai_shafik@yahoo.com
4
Salwa Ahmed
salwaa_fared@yahoo.com
5
Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
Oral Medicine, Periodontology, Oral Diagnosis and Radiology Department, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt
Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
Oral Medicine, Periodontology, Oral Diagnosis and Radiology Department, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt
Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
The aim of this study is to evaluate the efficacy of L-Carnitine and vitamin E alone or in combination on irradiated jaw bones in rats through radiographic assessment of the bone density and histopathologic examination of bone morphology. Forty male albino rats were divided randomly into 4 groups each of 10 rats. Group R represented the rats that received fractionated radiation dose (2 Gray per fraction) for one week. Groups LR, ER and LER represented the rats that received L-Carnitine 300mg/kg via intra peritoneal injection, vitamin E 40mg/kg via intramuscular injection or both daily for five days and then exposed to fractionated radiation 2 Gray day after day for one week after the last treatment, respectively. Data revealed a significant improvement of mandibular bone density in the groups received L-Carnitine, vitamin E alone or in combination as compared to the gamma-irradiated group. Moreover, the treated groups showed almost normal morphology of both mandibular bone and periodontal ligament in contrast to the gamma irradiated group where bone resorption and periodontal ligament degeneration and vacuolization were detected. In conclusion, gamma radiation had a deleterious effect on mandibular bone and periodontal ligament as indicated by decreased bone density and altered morphology. In addition, L-Carnitine and vitamin E treatment alone or in combination could, to a large extent, maintain the normal bone density and preserve the morphological architecture of mandibular bone and periodontal ligament.
https://ejrsa.journals.ekb.eg/article_25462_7f3b214e597269061005a0cab22e9c65.pdf
Gamma Irradiation
Vitamin E
L-carnitine
Mandible
periodontal ligament
bone density