Document Type : Original Article
Authors
1
Department of Radiation Biology, National Center for Radiation Research & Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt
2
Department of Radiation Microbiology, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Egypt (EAEA), Cairo, Egypt
Abstract
SINCE, gamma-radiation (γ-ray) is an efficient refinement approach to keep and potentiate the plant’s bioactive ingredients and biological activity, the influence of γ-ray (1, 3 and 5 kGy) on Rosa canina L. was investigated. Findings revealed that the ethanolic extract of irradiated and non-irradiated R. canina exert antimicrobial activity against all the pathogens tested to varying degrees. For all of the strains examined, the minimum inhibitory concentrations (MICs) varied from 16 to 64 μg ml-1. Interestingly, the strongest inhibitory activity was recorded when R. canina was subjected to 1.0 kGy. Furthermore, irradiation enhanced the total phenolic content, showinga maximum increase for R. canina irradiated at 1.0 kGy. The antioxidant activity was also increased when compared to non-irradiated R. canina. Accordingly, the anti-inflammatory efficacy of the ethanolic extract of R. canina subjected to 1.0 kGy (ERC) was determined on D-Galactosamine (D-GalN/γ-ray) in the rat model of acute hepatitis (AH). Theresults showed that ERC significantly improved liver function enzymes and healed the histopathological distortion of the hepatic architecture. Hepatic oxidative stress was repressed, as evidenced by the decrease in MDA, with a concomitant increase in the antioxidant status including SOD, CAT, GPx, GST activities and GSH levels. Additionally, ERC significantly reduced the inflammatory response of iNOS, NO, IL-1β, and TNF-α; promoted Nrf2 translocation to the nucleus and triggered the production of cytoprotective expressing HO-1 and NQO-1. It is concluded that γ-ray potentiates the hepato-protective effect of R. canina to combat acute hepatitis-associated inflammation via antioxidant effects, inducesdetoxification enzymes, and activates the Nrf2/HO-1 pathway.
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