Document Type : Original Article
1Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt
Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt
Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt
Applied Surfactant Laboratory, Egyptian Petroleum Research Institute, Nasr City, Cairo, Egypt
Background: 5-Fluorouracil (5-FU) is widely used in the treatment of cancer. The clinical use of 5-Flurouracil is constantly challenged by its poor selectivity and various side effects. Aim: The modification of 5-fluorouracil with Zinc oxide nanoparticles (NPs) and Cetrimonium bromide (CTAB) to enhance its antitumor activity was carried out. Material and Methods: The nanocomposite synthesis was evident by Scanning Electron Microscope (SEM) analysis, Transmission Electron Microscopy (TEM) analysis, X-Ray Diffraction (XRD) analysis and Fourier Transforms Infrared (FT-IR) spectroscopy. In addition to that the combined effect of ionizing gamma radiation with the modified 5-FU with Zinc oxide nanoparticles and Cetrimonium bromide (CTAB) was also investigated after 24 and 48 hr. The in vitro anticancer activity of nanocomposite (5-FU modified with Zinc oxide nano particles and CTAB) on liver cancer cells lines (HepG2), (HUH-7) and breast cancer cells (MCF-7) was investigated using MTT (3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay after 24 and 48 hr. Results: Nanocomposite exerted better in vitro anticancer activity against the previous human cancer cell lines comparable to the activity of 5-FU alone after 24 and 48 hr.
Conclusions: The effectiveness of 5-FU modified with Zinc oxide nanoparticles and CTAB as an antitumor drug is enhanced by the combination of Chemotherapy and Radiotherapy with Nanotechnology.