The Novel Modified 5-Fluorouracil with Zinc Oxide Nano-Particles and its Combined Effect with Gamma Radiations on Human Cancer Cell Lines

Hassan, N. A.; El-Dessouky, M.A.; Salah Eldin, A.M.; Korraa, S.S.; Elgazzar, Eman M.; Badawi, A.M.

doi:10.21608/ejrsa.2023.193748.1150

The Novel Modified 5-Fluorouracil with Zinc Oxide Nano-Particles and its Combined Effect with Gamma Radiations on Human Cancer Cell Lines

Document Type : Original Article

Authors

¹ 1Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt

² Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt

³ Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt

⁴ Applied Surfactant Laboratory, Egyptian Petroleum Research Institute, Nasr City, Cairo, Egypt

10.21608/ejrsa.2023.193748.1150

Abstract

Background: 5-Fluorouracil (5-FU) is widely used in the treatment of cancer. The clinical use of 5-Flurouracil is constantly challenged by its poor selectivity and various side effects. Aim: The modification of 5-fluorouracil with Zinc oxide nanoparticles (NPs) and Cetrimonium bromide (CTAB) to enhance its antitumor activity was carried out. Material and Methods: The nanocomposite synthesis was evident by Scanning Electron Microscope (SEM) analysis, Transmission Electron Microscopy (TEM) analysis, X-Ray Diffraction (XRD) analysis and Fourier Transforms Infrared (FT-IR) spectroscopy. In addition to that the combined effect of ionizing gamma radiation with the modified 5-FU with Zinc oxide nanoparticles and Cetrimonium bromide (CTAB) was also investigated after 24 and 48 hr. The in vitro anticancer activity of nanocomposite (5-FU modified with Zinc oxide nano particles and CTAB) on liver cancer cells lines (HepG2), (HUH-7) and breast cancer cells (MCF-7) was investigated using MTT (3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay after 24 and 48 hr. Results: Nanocomposite exerted better in vitro anticancer activity against the previous human cancer cell lines comparable to the activity of 5-FU alone after 24 and 48 hr.
Conclusions: The effectiveness of 5-FU modified with Zinc oxide nanoparticles and CTAB as an antitumor drug is enhanced by the combination of Chemotherapy and Radiotherapy with Nanotechnology.

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