Black Garlic Extract and /or Low Doses of Gamma Irradiation Ameliorates Hepatorenal functions in the Ehrlich Carcinoma bearing Mice model

Abd ElWahab, Safaa Abd El Wahab; Algeda, Fatma; Shedid, Shereen M; Ahmed, Hanafi

doi:10.21608/ejrsa.2025.356143.1179

Black Garlic Extract and /or Low Doses of Gamma Irradiation Ameliorates Hepatorenal functions in the Ehrlich Carcinoma bearing Mice model

Document Type : Original Article

Authors

¹ Food irradiation department

² Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt

³ radiation biology, atomic energy authority

⁴ National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt.

10.21608/ejrsa.2025.356143.1179

Abstract

Black garlic is a unique type of garlic that undergoes a specific processing method involving controlled levels of moisture and heat, resulting in a black color. It is known for its potential health benefits, particularly in preventing diseases such as cancer. Additionally, exposure to low doses of gamma radiation has been found to positively impact tumor regression and enhance immune responses. The current study aimed to evaluate the immunemodulatory effects of black garlic extract supplementation and/or low doses of gamma irradiation against Ehrlich carcinoma (EC)-induced hepatorenal complications in albino mice. On the seventh day after tumor inoculation, black garlic extracts (200 mg/kg body weight) were administered orally via gavage for 15 consecutive days, while the mice were also subjected to irradiation at 0.25 Gy twice a week for two weeks. The results showed that the implantation of Ehrlich carcinoma in mice increased oxidative stress in the liver and kidney tissues, leading to histopathological alterations. However, pretreatment with black garlic extract and/or low doses of gamma irradiation significantly improved most of the histopathological and biochemical parameters associated with EC inoculation. In conclusion, the administration of black garlic to the mice, either alone or in combination with low doses of gamma radiation, exhibited an antitumor effect. This was reflected by a reduction in tumor size, inhibition of systemic inflammation, improvement in liver and kidney function, modulation of lipid peroxidation, and enhancement of the antioxidant defense system in mice bearing EC.

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