Black Garlic Extract Polyphenols Constituents Adjuvant to Low Doses of Gamma Irradiation Exhibit Oxidative Stress on Murine Tumor

Ahmed, Neamat Hanafi; Shedid, Shereen; Abd ElWahab, Safaa Abd El Wahab; Rabia, Fatma

doi:10.21608/ejrsa.2025.333983.1177

Black Garlic Extract Polyphenols Constituents Adjuvant to Low Doses of Gamma Irradiation Exhibit Oxidative Stress on Murine Tumor

Document Type : Original Article

Authors

¹ Radiation Biology Department, National Center for radiation Research and Technology, Egyptian Atomic Energy Authority

² Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt

³ Food Irradiation Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt.

⁴ atomic energy authority

10.21608/ejrsa.2025.333983.1177

Abstract

Background: with precisely controlled degrees of moisture and heat the color of raw garlic turn black. Black garlic have a strong role in preventing cancer. Also, low doses of γ-radiation exposure induce a positive impact on tumor regression and immune response. Methodology: the present study evaluates the leverages of black garlic extract polyphenols (BGE) and/or low doses of γ-irradiation against Ehrlich (Eh) carcinoma-bearing mice. Results: the in-vitro cytotoxic effect test showed that, BGE (60 µg / ml) produced the death of 100% of Ehrlich Ascites Carcinoma (EAC) cells and 30% of cells appeared to burst. Subcutaneous transplantation of 2.5x106 EAC cells in female mice produced a solid Eh carcinoma. Via gavages 15 consecutive treatments of BGE (200mg/kg. b.w./day) and the subject of experimental animals to γ-radiation (0.25Gy x 2/ week) for two weeks from the 7th day of tumor inoculation recording that BGE and/or γ-radiation reduced tumor size significantly and markedly amended most alterations in biochemical parameters associated with Eh carcinoma inoculation. Conclusion: the BGE gavages to mice bearing Eh carcinoma, exhibited an antitumor effect reflected by reducing tumor size, inhibiting systemic inflammation, and augmenting the antioxidant defense system in Eh carcinoma-bearing mice.

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